Journal: Animal Bioscience

Article Title: Plant essential oil supplementation promotes growth and attenuates lipopolysaccharide (LPS)-induced acute liver injury through SIRT1/PGC-1α signaling pathway in nursery pigs

doi: 10.5713/ab.25.0066

Figure Lengend Snippet: Effect of dietary supplementation with plant essential oil (PEO) and coated plant essential oil (CEO) on the protein expression levels of SIRT1/PGC-1α signaling pathway in the liver of nursery piglets challenged by lipopolysaccharide (LPS). CON, control group, piglets fed the basal diet and injected with 100 μg/kg BW saline; LPS, piglets fed the basal diet and injected with LPS at a dose of 100 μg/kg body weight; LPS+PEO, piglets fed the basal diet supplemented with 500 mg/kg PEO and injected with LPS; LPS+CEO, piglets fed the basal diet supplemented with 500 mg/kg CEO and injected with LPS. a–c Means with different superscript differ significantly (p<0.05). PGC-1α, peroxisome proliferator-activated receptor γ coactivator 1α; SIRT1, silent information regulator 1.

Article Snippet: Following transfer, the membranes were blocked with 5% skimmed milk for 2 h. The membranes were then incubated overnight at 4°C with rabbit anti-PGC-1α monoclonal antibody (dilution 1:500; ABclonal), rabbit anti-SIRT1 monoclonal antibody (dilution 1:800; Abways), and rabbit anti-β-actin monoclonal antibody (dilution 1:5,000; Proteintech), respectively.

Techniques: Expressing, Control, Injection, Saline